Robust parameter design of human induced pluripotent stem cell differentiation protocols defines lineage‐specific induction of anterior‐posterior gut tube endodermal cells

by rss feed | Jan 15, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

We utilized robust parameter design (RPD) for establishing human induced pluripotent stem cell differentiation protocols, which led to the four distinctive endodermal cell induction, while increasing the efficiency of the multifactorial screening in 243‐fold (18 vs...

IGFBPs mediate IGF‐1’s functions in retinal lamination and photoreceptor development during pluripotent stem cell differentiation to retinal organoids

by rss feed | Jan 14, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

In order to investigate the role of insulin growth factor binding proteins (IGFBPs) in retinal organoids, human embryonic stem cells (hESCs) were differentiated to retinal organoids using three different conditions: with IGF‐1 individually or in combination with...

ERG1 plays an essential role in rat cardiomyocyte fate decision by mediating AKT signaling

by rss feed | Jan 11, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

ERG1 binds with Integrin β1 to activate the AKT pathway by recruiting and phosphorylating PI3K p85 and FAK, which promotes rat cardiomyocyte differentiation through mediating phosphorylation of GSK3β to upregulate the expression of both β‐catenin and Gata4, and...

miR‐183/96/182 cluster is an important morphogenetic factor targeting PAX6 expression in differentiating human retinal organoids

by rss feed | Dec 2, 2020 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

Inhibition of the miR‐183/96/182 cluster in human pluripotent stem cells leads to the generation of abnormal retinal organoids. Abstract MicroRNAs (miRNAs), a class of small, noncoding RNA molecules, represent important regulators of gene expression. Recent reports...

MiR‐184 directly targets Wnt3 in cardiac mesoderm differentiation of embryonic stem cells

by rss feed | Dec 2, 2020 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

MiR‐184 is critical for the differentiation of ES cells by directly targeting Wnt3, thereby inhibits the differentiation of ES cells into cardiac mesoderm, and finally affects the differentiation of cardiac mesoderm into cardiomyocytes. These findings not only...

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition

by rss feed | Nov 26, 2020 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

We generated human induced pluripotent stem cell‐derived alveolar type I (iAT1) cells in alveolar organoids (AOs) and showed that the transcriptomes of iAT1 cells were similar to those of primary AT1 cells. We generated iAT1 cells by inhibiting canonical Wnt signaling...