Cell cycle‐coupled changes in the level of reactive oxygen species support the proliferation of human pluripotent stem cells

by rss feed | Dec 5, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

The progression of the cell cycle in human pluripotent stem cells is coupled with the oscillation of the reactive oxygen species (ROS) level. The decrease in this level results in the disturbance of the regulation and progression of S-phase, which, in turn, cause the...

The cyclin‐like protein SPY1 overrides reprogramming induced senescence through EZH2 mediated H3K27me3

by rss feed | Dec 5, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds, Uncategorized

Reprogramming of normal fibroblasts to induced pluripotent stem cells is characterized by low efficiency due to the upregulation of tumor suppressors and reprogramming-induced senescence (RIS). Spy1 can be combined with classical pluripotency factors to override RIS...

Fluid shear stress promotes embryonic stem cell pluripotency via interplay between β‐catenin and vinculin in bioreactor culture

by rss feed | Apr 9, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

Schematic illustration of current study focusing on the effect of fluid shear stress on adherens junction proteins in murine embryonic stem cells. No shear stress in adherent culture retains β‐catenin in the adherens junction, whereas high shear stress in bioreactor...

Transplanted pluripotent stem cell‐derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration

by rss feed | Mar 6, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

This study assessed the ability of pluripotent stem cell derived cone photoreceptors to engraft in an end‐stage mouse model of photoreceptor degeneration model and restore vision. Our data demonstrate that pluripotent stem cell derived cone precursors can engraft into...

A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair generating improved genetically stable human induced pluripotent stem cells

by rss feed | Feb 23, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

Proposed clinical grade method to make better genetically stable human iPSC for clinical applications. CYCLIN D1 generated human iPSC have significantly improved genomic stability than current clinical grade methods that use C‐MYC, and thus clinically advantageous to...

A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair generating improved genetically stable human induced pluripotent stem cells

by rss feed | Feb 23, 2021 | EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS, News Feeds

Abstract A key challenge for clinical application of induced pluripotent stem cells (iPSC) to accurately model and treat human pathologies depends on developing a method to generate genetically stable cells to reduce long‐term risks of cell transplant therapy. Here,...